Purification of the lectin from Datura stramonium
نویسندگان
چکیده
منابع مشابه
Purification of the lectin from Datura stramonium.
The lectin from Datura stramonium can be inhibited by oligomers of N-acetylglucosamine. This property was exploited to purify the lectin by affinity chromatography on Sepharosefetuin. The purified lectin is a glycoprotein in having subunits of 40 000 and 45 000 mol.wt.
متن کاملCarbohydrate binding studies on the lectin from Datura stramonium seeds.
The carbohydrate-binding properties of the Datura stramonium seed lectin were studied by equilibrium dialysis, quantitative precipitation of natural and synthetic glycoproteins, and hapten inhibition of precipitation. The dimeric lectin (Mr = 86,000) possesses two carbohydrate-binding sites for N,N'N",N"'- tetraacetylchitotetritol /mol protein, with an apparent Ka = 8.7 X 10(3) M-1 at 4 degrees...
متن کاملPoisoning by Datura (Stramonium)— Recovery
partaken of a quantity of datura leaves, prepared in the form of curry, with rice, which he afterwards acknowledged to have voluntarily taken. On admission, he lay perfectly unconscious, with severe spasmodic muscular twitchings and stertorous breathing. Skin cool; pulse about 108?, feeble. Pupils dilated and insensible to the effects of light. The stomach pump was immediately introduced, but d...
متن کاملDatura stramonium poisoning in children.
Although substance abuse is fairly common among adolescents, poisoning from Datura stramonium (a broadleaf annual erect herb with spine-covered seed capsule) is uncommon in children and has not been reported in our locality. We present the case of two children admitted at the Children Emergency Room of a teaching hospital following ingestion of extract of Datura stramonium. They developed neuro...
متن کاملCarbohydrate binding properties of complex-type oligosaccharides on immobilized Datura stramonium lectin.
The carbohydrate binding specificity of Datura stramonium agglutinin was studied by analyzing the behavior of a variety of complex-type oligosaccharides on a D. Stramonium agglutinin-Sepharose column. Oligosaccharides which contain Gal beta 1----4GlcNAc-beta 1----4(Gal beta 1----GlcNAc beta 1----2)Man units are retarded in the column so long as the pentasaccharide unit is not substituted by oth...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1978
ISSN: 0264-6021
DOI: 10.1042/bj1751151